Indian Journal of Medical Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 144  |  Issue : 3  |  Page : 409--423

Transcriptome profiling of visceral adipose tissue in a novel obese rat model, WNIN/Ob & its comparison with other animal models


Siva Sankara Vara Prasad Sakamuri1, Uday Kumar Putcha2, Giridharan Nappan Veettil3, Vajreswari Ayyalasomayajula1 
1 Department of Biochemistry, National Institute of Nutrition (ICMR), Hyderabad, India
2 Department of Pathology, National Institute of Nutrition (ICMR), Hyderabad, India
3 Molecular Genetics Laboratory, National Center for Laboratory Animal Sciences (ICMR), Hyderabad, India

Correspondence Address:
Vajreswari Ayyalasomayajula
Department of Biochemistry, National Institute of Nutrition (ICMR), Jamai Osmania (PO), Hyderabad 500 007, Telangana
India

Background & objectives: Adipose tissue dysfunction in obesity is linked to the development of type 2 diabetes and cardiovascular diseases. We studied the differential gene expression in retroperitoneal adipose tissue of a novel obese rat model, WNIN/Ob, to understand the possible underlying transcriptional changes involved in the development of obesity and associatedcomorbidities in this model. Methods: Four month old, male WNIN/Ob lean and obese rats were taken, blood was collected and tissues were dissected. Body composition analysis and adipose tissue histology were performed. Global gene expression in retroperitoneal adipose tissue of lean and obese rats was studied by microarray using Affymetrix GeneChips. Results: One thousand and seventeen probe sets were downregulated and 963 probe sets were upregulated (more than two-fold) in adipose tissue of WNIN/Ob obese rats when compared to that of lean rats. Small nucleolar RNA (SnoRNA) made most of the underexpressed probe sets, whereas immune system-related genes werethe most overexpressed in the adipose tissues of obese rats. Genes coding for cytoskeletal proteinswere downregulated, whereas genes related to lipid biosynthesis were elevated in the adipose tissue of obese rats. Interpretation & conclusions: Majority of the altered genes and pathways in adipose tissue of WNIN/Ob obese rats were similar to the observations in other obese animal models and human obesity. Based on these observations, it is proposed that WNIN/Ob obese rat model may be a good model to study the mechanisms involved in the development of obesity and its comorbidities. Downregulation of SnoRNA appears to be a novel feature in this obese rat model.


How to cite this article:
Sakamuri SS, Putcha UK, Veettil GN, Ayyalasomayajula V. Transcriptome profiling of visceral adipose tissue in a novel obese rat model, WNIN/Ob & its comparison with other animal models.Indian J Med Res 2016;144:409-423


How to cite this URL:
Sakamuri SS, Putcha UK, Veettil GN, Ayyalasomayajula V. Transcriptome profiling of visceral adipose tissue in a novel obese rat model, WNIN/Ob & its comparison with other animal models. Indian J Med Res [serial online] 2016 [cited 2020 Aug 9 ];144:409-423
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=144;issue=3;spage=409;epage=423;aulast=Sakamuri;type=0