Indian Journal of Medical Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 144  |  Issue : 2  |  Page : 245--249

Use of hydrogen peroxide vapour & plasma irradiation in combination for quick decontamination of closed chambers


Devendra T Mourya1, Hamish C Shahani2, Pragya D Yadav1, Pradip V Barde3 
1 Maximum Containment Laboratory, Microbial Containment Complex, National Institute of Virology, Pune, India
2 Klenzaids Contamination Controls (P) Ltd. MIDC, Mumbai, India
3 Department of Virology, National Institute for Research in Tribal Health, Jabalpur, India

Correspondence Address:
Dr Devendra T Mourya
Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411 021, Maharashtra
India

Background & objectives: Various conventional methods such as gaseous, vapour and misting systems, fogging, manual spray and wipe techniques employing a number of chemical agents are used for decontamination of enclosed spaces. Among all these methods, use of aerosolized formaldehyde is the most preferred method due to cost-effectiveness and practical aspects. However, being extremely corrosive in nature generating very irritating fumes and difficulty in maintaining a high level of gas concentration, many laboratories prefer the vaporization of hydrogen peroxide (H 2 O 2 ) as an alternative. We present here the results of using H 2 O 2 vapour in combination with plasma irradiation for quick decontamination of closed chambers. Methods: The present study describes a decontamination method, using plasma irradiation in combination with H 2 O 2 (5%). Effect of plasma irradiation and H 2 O 2 on the viability of bacterial spores (Bacillus subtilis), Chikungunya and Kyasanur Forest Disease viruses was assessed. Results: Data suggest that with the combination of H 2 O 2 vapour and plasma irradiation, within short time (three minutes), decontamination of surfaces and space volume could be achieved. Although it showed damage of spores present on the strips, it did not show any penetration power. Interpretation & conclusions: The results were encouraging, and this method was found to be efficient for achieving surface sterilization in a short time. This application may be useful in laboratories and industries particularly, those working on clean facility concept following good laboratory and manufacturing practices.


How to cite this article:
Mourya DT, Shahani HC, Yadav PD, Barde PV. Use of hydrogen peroxide vapour & plasma irradiation in combination for quick decontamination of closed chambers.Indian J Med Res 2016;144:245-249


How to cite this URL:
Mourya DT, Shahani HC, Yadav PD, Barde PV. Use of hydrogen peroxide vapour & plasma irradiation in combination for quick decontamination of closed chambers. Indian J Med Res [serial online] 2016 [cited 2019 Aug 24 ];144:245-249
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=144;issue=2;spage=245;epage=249;aulast=Mourya;type=0