Indian Journal of Medical Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 143  |  Issue : 5  |  Page : 643--651

Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosa causing inhibition of biofilm formation & reduction of virulence


Parul Gupta, Sanjay Chhibber, Kusum Harjai 
 Department of Microbiology, Panjab University, Chandigarh, India

Correspondence Address:
Dr. Kusum Harjai
Department of Microbiology, BMS Block, Panjab University, Chandigarh 160 014
India

Background & objectives: Biofilms formed by pseudomonas aeruginosa lead to persistent infections. Use of antibiotics for the treatment of biofilm induced infection poses a threat towards development of resistance. Therefore, the research is directed towards exploring the property of antibiotics which may alter the virulence of an organism besides altering its growth. The aim of this study was to evaluate the role of subinhibitory concentration of ciprofloxacin (CIP) in inhibiting biofilm formation and virulence of P. aeruginosa. Methods: Antibiofilm potential of subinhibitory concentration of CIP was evaluated in terms of log reduction, biofilm forming capacity and coverslip assay. P. aeruginosa isolates (grown in the presence and absence of sub-MIC of CIP) were also evaluated for inhibition in motility, virulence factor production and quorum sensing (QS) signal production. Results: Sub-minimum inhibitory concentration (sub-MIC) of CIP significantly reduced the motility of P. aeruginosa stand and strain and clinical isolates and affected biofilm forming capacity. Production of protease, elastase, siderophore, alginate, and rhamnolipid was also significantly reduced by CIP. Interpretation & conclusions: Reduction in virulence factors and biofilm formation was due to inhibition of QS mechanism which was indicated by reduced production of QS signal molecules by P. aeruginosa in presence of subinhibitory concentration of CIP.


How to cite this article:
Gupta P, Chhibber S, Harjai K. Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosa causing inhibition of biofilm formation & reduction of virulence.Indian J Med Res 2016;143:643-651


How to cite this URL:
Gupta P, Chhibber S, Harjai K. Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosa causing inhibition of biofilm formation & reduction of virulence. Indian J Med Res [serial online] 2016 [cited 2019 Nov 17 ];143:643-651
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=5;spage=643;epage=651;aulast=Gupta;type=0