Indian Journal of Medical Research

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 140  |  Issue : 5  |  Page : 665--671

Time dependent enhanced resistance against antibiotics & metal salts by planktonic & biofilm form of Acinetobacter haemolyticus MMC 8 clinical isolate


Sharvari Vijaykumar Gaidhani1, Aartee Vishnu Raskar1, Sharmishtha Poddar2, Shriya Gosavi2, Praveen Kishore Sahu1, Karishma Rajendra Pardesi2, Shobhana V Bhide3, Balu Ananada Chopade1 
1 Institute of Bioinformatics & Biotechnology, University of Pune, Pune, India
2 Department of Microbiology, University of Pune, Pune, India
3 Department of Chemistry, University of Pune, Pune, India

Correspondence Address:
Balu Ananada Chopade
Department of Microbiology, University of Pune, Pune 411 007, Maharashtra
India

Background & objectives: Available literature shows paucity of reports describing antibiotic and metal resistance profile of biofilm forming clinical isolates of Acinetobacter haemolyticus. The present study was undertaken to evaluate the antibiotic and metal resistance profile of Indian clinical isolate of A. haemolyticus MMC 8 isolated from human pus sample in planktonic and biofilm form. Methods: Antibiotic susceptibility and minimum inhibitory concentration were determined employing broth and agar dilution techniques. Biofilm formation was evaluated quantitatively by microtiter plate method and variation in complex architecture was determined by scanning electron microscopy. Minimum biofilm inhibiting concentration was checked by Calgary biofilm device. Results: Planktonic A. haemolyticus MMC 8 was sensitive to 14 antibiotics, AgNO 3 and HgC1 2 resistant to streptomycin and intermediately resistant to netilmycin and kanamycin. MMC 8 exhibited temporal variation in amount and structure of biofilm. There was 32 - 4000 and 4 - 256 fold increase in antibiotic and metal salt concentration, respectively to inhibit biofilm over a period of 72 h as against susceptible planktonic counterparts. Total viable count in the range of 10 5 -10 6 cfu / ml was observed on plating minimum biofilm inhibiting concentration on Muller-Hinton Agar plate without antimicrobial agents. Biofilm forming cells were several folds more resistant to antibiotics and metal salts in comparison to planktonic cells. Presence of unaffected residual cell population indicated presence of persister cells. Interpretation & conclusions: The results indicate that biofilm formation causes enhanced resistance against antibiotics and metal salts in otherwise susceptible planktonic A. haemolyticus MMC 8.


How to cite this article:
Gaidhani SV, Raskar AV, Poddar S, Gosavi S, Sahu PK, Pardesi KR, Bhide SV, Chopade BA. Time dependent enhanced resistance against antibiotics & metal salts by planktonic & biofilm form of Acinetobacter haemolyticus MMC 8 clinical isolate.Indian J Med Res 2014;140:665-671


How to cite this URL:
Gaidhani SV, Raskar AV, Poddar S, Gosavi S, Sahu PK, Pardesi KR, Bhide SV, Chopade BA. Time dependent enhanced resistance against antibiotics & metal salts by planktonic & biofilm form of Acinetobacter haemolyticus MMC 8 clinical isolate. Indian J Med Res [serial online] 2014 [cited 2020 Feb 29 ];140:665-671
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2014;volume=140;issue=5;spage=665;epage=671;aulast=Gaidhani;type=0