Indian Journal of Medical Research

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 140  |  Issue : 4  |  Page : 513--519

Double positive CD4+CD8+ T cells: key suppressive role in the production of autoantibodies in systemic lupus erythematosus


Yongkang Wu1, Bei Cai1, Weihua Feng1, Bin Yang1, Zhuochun Huang1, Chuan Zuo2, Lanlan Wang1 
1 Department of Laboratory Medicine, West China Hospital affiliated Sichuan University, Chengdu, China
2 Department of Rheumatology, West China Hospital affiliated Sichuan University, Chengdu, China

Correspondence Address:
Lanlan Wang
Department of Laboratory Medicine, West China Hospital affiliated Sichuan University, 37 Guoxue Xiang, Chengdu, 610041, Sichuan
China

Background & objectives: The presence of CD4+CD8+ (double positive) T cells (DPT) in the target organs of several autoimmune diseases has been reported. The aim of this study was to investigate the pathogenic role of DPT in systemic lupus erythematosus (SLE). Methods: A total of 175 SLE cases and 125 matched healthy controls were investigated for CD3+, CD4+, CD8+ lymphocytes and DPT by flow cytometry. Serum samples from SLE patients and controls were tested for antinuclear antibody (ANA), anti-double strain deoxyribonucleic acid (anti-dsDNA), anti-U1 ribonucleoprotein (anti-U1 RNP), anti-sjogren syndrome A (anti-SSA), anti-ribosomal P protein (anti-rib-P), anti-Smith (anti-Sm), anti-Sjogren syndrome B (anti-SSB), complement 3 (C3) and complement 4 (C4). Results: The DPT median and 5-95 per cent range of SLE cases and healthy controls were 0.50 [0.10-2.60] and 0.80 [0.20-2.74] respectively (P<0.001). SLE patients were divided into a ≥1:1000 subgroup and a <1:1000 subgroup according to the ANA titre. The DPT of the former subgroup was significantly lower than that of the latter (P=0.032). The DPT medians of positive subgroups with anti-dsDNA (P<0.001), anti-U1RNP (P=0.018), anti-SSA (P=0.021) or anti-rib-P (P=0.039) were also significantly lower than the negative subgroups. Likewise, DPT was significantly lower in SLE subgroups with low concentration of C3 or C4 than those with high concentration (P<0.006). Interpretation & conclusions: Our findings show that the DPT cells may play a key suppressive role in the production of autoantibodies in SLE. Direct evidence that DPT regulates the pathogenesis of SLE needs to be investigated in future work.


How to cite this article:
Wu Y, Cai B, Feng W, Yang B, Huang Z, Zuo C, Wang L. Double positive CD4+CD8+ T cells: key suppressive role in the production of autoantibodies in systemic lupus erythematosus.Indian J Med Res 2014;140:513-519


How to cite this URL:
Wu Y, Cai B, Feng W, Yang B, Huang Z, Zuo C, Wang L. Double positive CD4+CD8+ T cells: key suppressive role in the production of autoantibodies in systemic lupus erythematosus. Indian J Med Res [serial online] 2014 [cited 2019 Jul 18 ];140:513-519
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2014;volume=140;issue=4;spage=513;epage=519;aulast=Wu;type=0