Year : 2014 | Volume
: 139 | Issue : 6 | Page : 913--920
Production of a compound against methicillin resistant Staphylococcus aureus (MRSA) from Streptomyces rubrolavendulae ICN3 & its evaluation in zebrafish embryos
Rajaretinam Rajesh Kannan, Appadurai Muthamil Iniyan, Samuel Gnana Prakash Vincent
Centre for Nanoscience & Nanotechnology (CNSNT), Sathyabama University, Chennai; International Centre for Nanobiotechnology, Centre for Marine Science & Technology, Manonmaniam Sundaranar University, Rajakkamangalam, India
Background & objectives: Antibiotic resistance in pathogens has become a serious problem worldwide. Therefore, the search for new antibiotics for drug resistanct pathogens is an important endeavor. The present study deals with the production of anti-methicillin resistant Staphylococcus aureus (MRSA) potential of Streptomyces rubrolavendulae ICN3 and evaluation of anti-MRSA compound in zebrafish embryos.
Methods: The antibiotic production from S. rubrolavendulae ICN3 was optimized in solid state fermentation and extracted. The antagonistic activity was confirmed against MRSA and purified in silica gel column and reverse phase - HPLC with an absorption maximum at 215 nm. Minimal inhibitory concentration of the compound was determined by broth microdilution method. Zebrafish embryos were used to evaluate the extract/compound for its minimal inhibition studies, influences on heart beat rates, haematopoietic blood cell count and lethal dose values.
Results: Streptomyces rubrolavendulae ICN3 showed potent antagonistic activity against MRSA with a zone of 42 mm. The minimum inhibitory concentration was calculated as 500 μg/ml of the crude extract and the purified C23 exhibited 2.5 μg/ml in in vitro assay. The LC 50 value of the anti MRSA compound C23 was calculated as 60.49 μg/ml and the MRSA treated embryos survived in the presence of purified compound C23 at a dose of 10 μg/ml.
Interpretation & conclusions: Our results suggested that the compound was potent with less toxic effects in zebrafish embryonic model system for MRSA infection. Further structural evaluation and analysis in higher mammalian model system may lead to a novel drug candidate for drug resistant Staphylococcus aureus.
Samuel Gnana Prakash Vincent
Coordinator, International Centre for Nanobiotechnology (ICN) Centre for Marine Science & Technology (CMST), Manonmaniam Sundaranar University Rajakkamangalam 629 502, Kanyakumari District, TN
|How to cite this article:|
Kannan RR, Iniyan AM, Vincent SG. Production of a compound against methicillin resistant Staphylococcus aureus (MRSA) from Streptomyces rubrolavendulae ICN3 & its evaluation in zebrafish embryos.Indian J Med Res 2014;139:913-920
|How to cite this URL:|
Kannan RR, Iniyan AM, Vincent SG. Production of a compound against methicillin resistant Staphylococcus aureus (MRSA) from Streptomyces rubrolavendulae ICN3 & its evaluation in zebrafish embryos. Indian J Med Res [serial online] 2014 [cited 2020 Aug 13 ];139:913-920
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2014;volume=139;issue=6;spage=913;epage=920;aulast=Kannan;type=0