Indian Journal of Medical Research

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 138  |  Issue : 1  |  Page : 104--110

Effect of growth factors (BMP-4/7 & bFGF) on proliferation & osteogenic differentiation of bone marrow stromal cells


Shaohui Yuan, Qi Pan, Chun Jiang Fu, Zhenggang Bi 
 Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China

Correspondence Address:
Zhenggang Bi
Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin 150001
P.R. China

Background & objectives: BMP (bone morphogenetic protein)-4/7 and bFGF (basic fibroblast growth factor) significantly promote the osteogenic activity and the proliferation of rabbit BMSCs (bone marrow stromal cells), respectively. However, their synergistic effects on the proliferation and the differentiation of BMSCs remain unclear. In the present study, the effects of bFGF and BMP-4/7 were investigated on the proliferation and the differentiation of rat BMSCs in vitro. Methods: BMSCs were isolated from New Zealand white rabbits and cultured to the third passage. The samples were divided into five groups according to the material implanted: (A) 80 ng/ml BMP-4/7; (B) 80 ng/ml bFGF; (C) 30 ng/ml BMP-4/7 and 30 ng/ml bFGF; (D) 50 ng/ml BMP-4/7 and 50 ng/ml bFGF; and (E) 80 ng/ml BMP-4/7 and 80 ng/ml bFGF. Cell proliferation was analyzed using methyl thiazolyl tetrazolium (MTT) assay. Alkaline phosphatase activity and osteocalcin (OC) dynamics were also measured. Results: BMP-4/7 alone significantly (P<0.05) promoted the proliferation of BMSCs. At the same time, it also promoted or inhibited the osteogenic differentiation of BMSCs. The synergistic effects of BMP-4/7 and bFGF significantly promoted both the proliferation and the osteogenic differentiation of BMSCs. The treatment of the synergistic effects was dose and time dependent. Interpretation & conclusions: A rational combination of BMP-4/7 and bFGF can promote the proliferation and the osteogenic differentiation of BMSCs. In addition, the synergistic functions are effective.


How to cite this article:
Yuan S, Pan Q, Fu CJ, Bi Z. Effect of growth factors (BMP-4/7 & bFGF) on proliferation & osteogenic differentiation of bone marrow stromal cells.Indian J Med Res 2013;138:104-110


How to cite this URL:
Yuan S, Pan Q, Fu CJ, Bi Z. Effect of growth factors (BMP-4/7 & bFGF) on proliferation & osteogenic differentiation of bone marrow stromal cells. Indian J Med Res [serial online] 2013 [cited 2020 Feb 22 ];138:104-110
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2013;volume=138;issue=1;spage=104;epage=110;aulast=Yuan;type=0