Indian Journal of Medical Research

REVIEW ARTICLE
Year
: 2006  |  Volume : 124  |  Issue : 4  |  Page : 385--398

Applications of real-time PCR technology to mycobacterial research


D Parashar, DS Chauhan, VD Sharma, VM Katoch 
 National JALMA Institute for Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India

Correspondence Address:
V M Katoch
National JALMA Institute for Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India

Real-time polymerase chain reaction (PCR) is being used worldwide for various research purposes. In this review we discuss the principles of real-time PCR, different methodologies and chemistries available and their applications in mycobacterial research. This technology allows for the direct detection of PCR product during the exponential phase of the reaction. Being a very powerful, accurate, rapid and sensitive method, this technique holds immense promise for detection of mycobacteria, differentiation of mycobacterial species, quantification of mycobacterial load and detection of drug resistance in mycobacterial infection.


How to cite this article:
Parashar D, Chauhan D S, Sharma V D, Katoch V M. Applications of real-time PCR technology to mycobacterial research.Indian J Med Res 2006;124:385-398


How to cite this URL:
Parashar D, Chauhan D S, Sharma V D, Katoch V M. Applications of real-time PCR technology to mycobacterial research. Indian J Med Res [serial online] 2006 [cited 2020 Aug 12 ];124:385-398
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2006;volume=124;issue=4;spage=385;epage=398;aulast=Parashar;type=0