Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
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ORIGINAL ARTICLE
Year : 2019  |  Volume : 150  |  Issue : 3  |  Page : 254-260

Diagnostic ability of real-time quantitative polymerase chain reaction versus immunohistochemistry for Ki-67 assessment in breast cancer: An Indian perspective


1 Department of Laboratory & Transfusion Services; Department of Research; Department of Molecular Diagnostics & Cell Biology, Rajiv Gandhi Cancer Institute & Research Centre, Delhi, India
2 Department of Molecular Diagnostics & Cell Biology, Rajiv Gandhi Cancer Institute & Research Centre, Delhi, India
3 Department of Laboratory & Transfusion Services, Rajiv Gandhi Cancer Institute & Research Centre, Delhi, India
4 Department of Research, Rajiv Gandhi Cancer Institute & Research Centre, Delhi, India

Correspondence Address:
Dr Prerna Chadha
Department of Laboratory & Transfusion Services, Rajiv Gandhi Cancer Institute & Research Centre, Rohini, Sector 5, Delhi 110 085
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmr.IJMR_644_18

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Background & objectives: Breast cancer is the most common cancer of women. Inferior prognosis in some patients has been attributed to the higher proliferative capability of the tumour. Immunohistochemistry (IHC) for Ki-67, despite being a simple and cost-effective method, has not become a valid tool to evaluate this biomarker. This is ascribed to variation in pre-analytical and analytical techniques, variable expression, hotspot distribution and inter-and intra-observer inconsistency. This study was aimed at defining the analytical and clinical validity of real-time quantitative polymerase chain reaction (RT-qPCR) as an alternative to IHC evaluation. Methods: This study included a total of 109 patients with invasive breast cancers. Ki-67 IHC visual assessment was compared with the mRNA value determined by RT-qPCR. Concordance between both the methods was assessed. Receiver operating characteristic (ROC) curve analysis and Cohen's kappa value with intraclass correlation were performed. Results: The threshold value for Ki-67 by RT-qPCR obtained by ROC curve was 22.23 per cent, which was used to divide breast cancer cases into high proliferative and low proliferative groups. A significant correlation was observed between both the breast cancer groups formed using RT-qPCR threshold as well as median laboratory value of Ki-67 labelling index by IHC. Interpretation & conclusions: The study results showed a significant correlation between the two methods. While IHC is subject to technical and interpretative variability, RT-qPCR may offer a more objective alternative.


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