|Year : 2019 | Volume
| Issue : 1 | Page : 92-95
Serovar diversity of Salmonella among poultry
Yashwant Kumar, Varun Singh, Gulshan Kumar, Naveen Kumar Gupta, Ajay Kumar Tahlan
National Salmonella & Escherichia Centre, Central Research Institute, Kasauli, India
|Date of Submission||13-Nov-2017|
|Date of Web Publication||30-Sep-2019|
Dr Yashwant Kumar
National Salmonella & Escherichia Centre, Central Research Institute, Kasauli 173 204, Himachal Pradesh
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background & objectives: Salmonellosis due to the consumption of contaminated poultry products is a well-known public health concern, and assessing the distribution of Salmonella serovars among poultry becomes important for better prevention and control. The objective of the present study was to assess the distribution of Salmonella serovars among poultry.
Methods: The isolates received at National Salmonella and Escherichia Centre during 2011-2016 were subjected to biochemical identification, followed by serological characterization to identify the Salmonella serovars, and the data were presented to exhibit the distribution of Salmonella serovars among poultry.
Results: Salmonella was found to be present in poultry in all the regions included in the study. Salmonella Typhimurium, S. Gallinarum and S. Enteritidis were the most prevalent serovars accounting for 96.2 per cent of isolates. Salmonella was identified in poultry from all major egg-producing and egg-consuming States. Other serovars which were scantly identified included S. Infantis (2.7%), S. Montevideo (0.64%), S. Newport (0.26%) and S. Pullorum (0.13%).
Interpretation & conclusions: Diverse distribution of Salmonella serovars in poultry in India, with known potential to infect human population and/or other poultry flocks, requires urgent nationwide stringent control measures.
Keywords: Distribution - poultry - Salmonella - serovars
|How to cite this article:|
Kumar Y, Singh V, Kumar G, Gupta NK, Tahlan AK. Serovar diversity of Salmonella among poultry. Indian J Med Res 2019;150:92-5
Salmonellosis More Details has become a major public health concern worldwide and emerged as an important food-borne disease resulting in considerable public health and economic burden,. Salmonellosis is generally acquired through food-borne exposure, although direct contact with infected animals has also been reported,.
Contaminated poultry products, especially undercooked meat and raw eggs, have been reported as important sources of food-borne salmonellosis due to non-typhoidal Salmonella More Details,. India's poultry industry has got transformed from a mere backyard activity into a major commercial activity in just four decades and placed India as the world's third largest egg producer and the seventh largest producer of broiler. Different Salmonella serovars have been reported from poultry in several localized studies in different parts of the country earlier,,,,. However, the diversity of Salmonella serovars among poultry needs to be determined from time to time to assess the distribution trends. This may help veterinary and public health authorities in formulation of prevention strategies to control salmonellosis in poultry flocks to reduce economic losses which may further help to hinder the transmission of these serovars to humans. Therefore, an effort was made to generate and compile data on the distribution of Salmonella serovars among poultry.
| Material & Methods|| |
Bacterial isolates:Seven hundred and seventy eight suspected Salmonella isolates from poultry origin received at National Salmonella and Escherichia More Details Centre (NSEC), Central Research Institute, Kasauli, India, from various research, veterinary and academic institutes throughout the country, during January 2011 to October 2016 constituted the material for the study.
Bacterial identification and serotyping: Bacterial isolates were identified on the basis of culture characteristics, Gram staining, and biochemical tests. The isolates confirmed as Salmonella were further subjected to serotyping using an array of pooled and factor Salmonella antisera (Statens Serum Institute, Copenhagen, Denmark; Denka Seiken Co. Ltd., Tokyo, Japan).
| Results & Discussion|| |
Salmonellae were identified on the basis of characteristic biochemical results with catalase-positive, oxidase-negative, motility-positive (except Salmonella Gallinarum and S. Pullorum), glucose-positive, lactose-negative, mannitol-positive, sucrose-negative, salicin-negative, indole-negative, methyl red-positive, Voges-Proskauer negative, citrate utilization positive and H2S positive. S. Gallinarum and S. Pullorum were differentiated on the basis of gas production, dulcitol fermentation, maltose fermentation, and ornithine decarboxylation. Seven serovars were identified with S. Gallinarum (43.7%), being the most frequent, followed by S. Enteritidis (30.6%), S. Typhimurium (21.9%), S. Infantis (2.7%), S. Montevideo (0.64%), S. Newport (0.26%) and S. Pullorum (0.13%). Majority of identified Salmonella isolates were from poultry meat (71%), followed by blood (17.7%), faeces (7.7%) and eggs (3.6%) [Table 1]. The serovars exhibiting high proportion, namely S. Gallinarum, S. Typhimurium and S. Enteritidis, were found to be distributed uniformly in almost all States [Table 2].
|Table 1: Distribution of Salmonella serovars in various poultry specimens|
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|Table 2: Distribution of Salmonella serovars from poultry received at National Salmonella and Escherichia Centre from different States|
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Salmonellosis is one of the most common infectious diseases in animals and one of the major causes of food poisoning in humans. Food-borne infections caused by Salmonella serovars mainly through contaminated meat, eggs, and egg products occur at high frequency in industrialized nations and developing countries,,,. Salmonella infections in poultry are also very common,,,,, which not only leads to heavy economic loss to egg and broiler producers and people associated with poultry industry but also poses a threat of transmission of salmonellae to human beings.
In India, data on the distribution of Salmonella serovars in poultry have been generated at various localized geographical areas during different time periods,,,,.
S. Gallinarum, the causative agent of fowl typhoid, and a leading cause of morbidity and mortality in commercial poultry resulting in significant economic losses to the poultry farmers, was found to be in the highest proportions during 2011-2016, contributing 43.7 per cent of the total Salmonella isolates of poultry origin. S. Enteritidis and S. Typhimurium which are not only known to infect poultry but also act as potential agents of human gastroenteritis, were found to be the second and third most prevalent serovars during the study period. Outbreaks of Salmonella food poisoning due to the consumption of contaminated eggs, egg products or meat have been documented,,.
Due to increasing share of poultry industry in Indian economy and more concentration of poultry farms near human habitats, with known public health concerns for salmonellosis of poultry origin, well-planned surveillance and control programmes at national level with regular surveillance of Salmonella and stringent biosecurity measures in poultry industry may result in significant reductions in its prevalence in polutry,,. Considering the wide distribution of different Salmonella serovars in poultry, interventions from public health authorities and policymakers are required to control the spread of salmonellae. The strategies should include stringent farm management programmes using interventions at multiple stages of egg production including storage at lower temperatures and implementation of stringent control and food standards in the meat industry. Although studies have been conducted in different geographic areas of the country to assess the distribution of Salmonella serovars among poultry, there is a need for an integrated national-level surveillance programme including all States for delineating the diversity of Salmonella serovars among poultry.
Acknowledgment: Authors thank the Heads of all the laboratories who referred Salmonella isolates to National Salmonella & Escherichia Centre. The technical assistance of Shri Gian Chand Kashav is acknowledged.
Financial support & sponsorship: None.
Conflicts of Interest: None.
| References|| |
Majowicz SE, Musto J, Scallan E, Angulo FJ, Kirk M, O'Brien SJ, et al.
The global burden of nontyphoidal Salmonella
gastroenteritis. Clin Infect Dis
Ao TT, Feasey NA, Gordon MA, Keddy KH, Angulo FJ, Crump JA. Global burden of invasive nontyphoidal Salmonella
disease, 2010(1). Emerg Infect Dis
Centers for Disease Control and Prevention. Vital signs: Incidence and trends of infection with pathogens transmitted commonly through food – Foodborne diseases active surveillance network, 10 U.S. sites, 1996-2010. Morb Mortal Wkly Rep
Antunes P, Mourão J, Campos J, Peixe L. Salmonellosis: The role of poultry meat. Clin Microbiol Infect
Hennessy TW, Cheng LH, Kassenborg H, Ahuja SD, Mohle-Boetani J, Marcus R, et al.
Egg consumption is the principal risk factor for sporadic Salmonella
serotype Heidelberg infections: A case-control study in foodNet sites. Clin Infect Dis
(Suppl 3) : S237-43.
Barua H, Biswas PK, Talukder KA, Olsen KE, Christensen JP. Poultry as a possible source of non-typhoidal Salmonella enterica
serovars in humans in Bangladesh. Vet Microbiol
Chatterjee RN, Rajkumar U. An overview of poultry production in India. Indian J Anim Health
Arora D, Kumar S, Singh D, Jindal N, Mahajan NK. Isolation, characterization and antibiogram pattern of Salmonella
from poultry in parts of Haryana, India. Adv Anim Vet Sci
Rajagopal R, Mini M. Outbreaks of salmonellosis in three different poultry farms of Kerala, India. Asian Pac J Trop Biomed
Mir IA, Kashyap SK, Maherchandani S. Isolation, serotype diversity and antibiogram of Salmonella enterica
isolated from different species of poultry in India. Asian Pac J Biomed
Samanta I, Joardar SN, Das PK, Sar TK, Bandyopadhyay S, Dutta TK, et al
. Prevalence and antibiotic resistance profiles of Salmonella
serotypes isolated from backyard poultry flocks in West Bengal, India. J Appl Poul Res
Murugadas V, Visnuvinayagam S, Purushothaman V, Prabhakar TG, Prabhahar P, Ventakaraman K. Prevalence of chicken host and non-host adapted Salmonella
in retail outlet of Chennai, India. Asian J Ani Vet Adv
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Group 5: Facultatively anaerobic Gram negative rods. In: Holt GJ, editor. Bergey manual of determinative bacteriology
. Baltimore, MD: The Williams & Wilkins Co.; 1994. p. 175-289.
Grimont PAD, Weill FX. Antigenic formulae of the Salmonella
serovars. WHO Collaborating Centre for Reference and Research on Salmonella
ed. France: Institute Pasteur; 2007. Available from: https://www.pasteur.fr/sites/default/files/veng_0.pdf
, accessed on October 10, 2017.
Gantois I, Ducatelle R, Pasmans F, Haesebrouck F, Gast R, Humphrey TJ, et al.
Mechanisms of egg contamination by Salmonella
enteritidis. FEMS Microbiol Rev
Jarquin C, Alvarez D, Morales O, Morales AJ, López B, Donado P, et al. Salmonella
on raw poultry in retail markets in Guatemala: Levels, antibiotic susceptibility, and serovar distribution. J Food Prot
Issa Y, Abu-Rayyan A, Hemidat S. Prevalence of Salmonella
in different poultry and meat food products in Hebron district: A prevalence study. Lancet
Adeyanju GT, Ishola O. Salmonella
and Escherichia coli
contamination of poultry meat from a processing plant and retail markets in Ibadan, Oyo state, Nigeria. Springerplus
Prakash B, Krishnappa G, Muniyappa L, Kumar SB. Epidemiological characterization of avian Salmonella enterica
serovar infections in India. Int J Poul Sci
Foley SL, Nayak R, Hanning IB, Johnson TJ, Han J, Ricke SC. Population dynamics of Salmonella enterica
serotypes in commercial egg and poultry production. Appl Environ Microbiol
Selvaraj R, Das R, Ganguly S, Mukhopadhayay SK. Molecular characterization of Salmonella
spp. isolated and identified from chicken samples. Int J Pharm Res Bio Sci
Renu R, Yadav AS, Tripathi V, Singh RP. Salmonella
occurrence in chicken eggs and environmental samples and their sero-prevalence in laying hens. Indian J Anim Sci
Ramya P, Madhavarao T, Rao LV. Study on the incidence of Salmonella
enteritidis in poultry and meat samples by cultural and PCR methods. Vet World
Priyantha MAR. An overview: Vaccination to control fowl typhoid in commercial layers, Sri Lanka. Wayamba J Anim Sci
Kumar T, Mahajan NK, Rakha NK. Epidemiology of fowl typhoid in Haryana, India. World Poult Sci J
Dutta P, Borah MK, Gangil R, Singathia R. Gross/histopathological impact of Salmonella
Gallinarum isolated from layer chickens in Jaipur and their antibiogram assay. Int J Adv Vet Sci Technol
Dyda A, Hundy R, Moffatt CR, Cameron S. Outbreak of Salmonella
Typhimurium 44 related to egg consumption. Commun Dis Intell Q Rep
Wegener HC, Hald T, Lo Fo Wong D, Madsen M, Korsgaard H, Bager F, et al. Salmonella
control programs in Denmark. Emerg Infect Dis
Maijala R, Ranta J, Seuna E, Pelkonen S, Johansson T. A quantitative risk assessment of the public health impact of the Finnish Salmonella
control program for broilers. Int J Food Microbiol
Van Der Fels-Klerx HJ, Jacobs-Reitsma WF, Van Brakel R, Van Der Voet H, Van Asselt ED. Prevalence of Salmonella
in the broiler supply chain in the Netherlands. J Food Prot
[Table 1], [Table 2]