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ORIGINAL ARTICLE
Year : 2019  |  Volume : 149  |  Issue : 2  |  Page : 240-246

Molecular characterization & epidemiology of carbapenem-resistant Acinetobacter baumannii collected across India


1 Department of Clinical Microbiology, Christian Medical College, Vellore, India
2 Department of Lab Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India
3 Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
4 Department of Medical Microbiology, Postgraduate Institute of Medical Education & Research, Chandigarh, India
5 Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India
6 Division of Epidemiology & Communicable Diseases, Indian Council of Medical Research, New Delhi, India
7 Department of Surgical Intensive Care Unit, Christian Medical College, Vellore, India
8 Department of Medicine, Christian Medical College, Vellore, India

Correspondence Address:
Dr Balaji Veeraraghavan
Department of Clinical Microbiology, Christian Medical College, Vellore 632 004, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmr.IJMR_2085_17

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Background & objectives: Acinetobacter baumannii is an opportunistic pathogen responsible for causing nosocomial infections. A. baumannii develops resistance to various antimicrobial agents including carbapenems, thereby complicating the treatment. This study was performed to characterize the isolates for the presence of various β-lactamases encoding genes and to type the isolates to compare our clones with the existing international clones across five centres in India. Methods: A total 75 non-repetitive clinical isolates of A. baumannii from five different centres were included in this study. All the isolates were confirmed as A. baumannii by bl aOXA-51-likePCR. Multiplex PCR was performed to identify the presence of extended spectrum β-lactamases (ESBL) and carbapenemases. Multilocus sequence typing was performed to find the sequence type (ST) of the isolates. e-BURST analysis was done to assign each ST into respective clonal complex. Results: blaOXA-51-likewas present in all the 75 isolates. The predominant Class D carbapenemase was blaOXA-23-likefollowed by Class B carbapenemase, blaNDM-like. Class A carbapenemase was not observed. blaPER-likewas the predominant extended spectrum β-lactamase. ST-848, ST-451 and ST-195 were the most common STs. Eight-novel STs were identified. e-BURST analysis showed that the 75 A. baumannii isolates were clustered into seven clonal complexes and four singletons, of which, clonal complex 208 was the largest. Interpretation & conclusions: Most of the isolates were grouped under clonal complex 208 which belongs to the international clonal lineage 2. High occurrence of ST-848 carrying blaOXA-23-likegene suggested that ST-848 could be an emerging lineage spreading carbapenem resistance in India.


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