Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
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ORIGINAL ARTICLE
Year : 2018  |  Volume : 147  |  Issue : 5  |  Page : 507-512

Molecular characterization of hepatitis A virus from children hospitalized at a tertiary care centre in northwest India


1 Advanced Basic Sciences & Clinical Research Laboratory, Department of Microbiology & Immunology, Sawai Man Singh Medical College, Jaipur, India
2 School of Life & Basic Sciences, Jaipur National University, Jaipur, India

Correspondence Address:
Dr Bharti Malhotra
Advanced Basic Sciences & Clinical Research Laboratory, Department of Microbiology & Immunology, SMS Medical College, Jawahar Lal Nehru Marg, Jaipur 302 004, Rajasthan
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmr.IJMR_1910_15

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Background & objectives: Hepatitis A virus (HAV) infection is a major cause of childhood hepatitis, prevalent worldwide. HAV is classified into seven genotypes I-VII; genotypes III and I are the most common among humans. The present work was carried out to identify the genotypes prevalent in children suspected to have acute viral hepatitis (AVH), hospitalized at a tertiary care centre in northwest India. Methods: A total of 1269 blood samples from children (0-15 yr of age) clinically suspected of viral hepatitis were screened for anti-HAV IgM. Acute phase serum was processed for RNA extraction and amplified by nested polymerase chain reaction (PCR) followed by sequencing of representative samples. Results: Among the 1269 samples tested, 642 (50.59%) were positive for anti-HAV IgM; among the positive samples, 171 patients having a history of less than seven days were tested by PCR, of whom 141 (82.45%) were found to be PCR positive. Nucleotide sequencing of a representative 44 samples showed high homology; all the samples were found to be of genotype IIIA. Interpretation & conclusions: Hepatitis A was prevalent during July to September and in predominantly children less than five years age. Only genotype IIIA was detected in all the samples.


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