Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
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Year : 2018  |  Volume : 147  |  Issue : 4  |  Page : 391-399

Detection of parvovirus B19 in selected high-risk patient groups & their phylogenetic & selection analysis

1 Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital & Research Centre, Vellore, India
2 Department of Microbiology, King George Medical University, Lucknow, India
3 Kusuma School of Biological Sciences, Indian Institute of Technology, New Delhi, India
4 Department of Microbiology, Sri Ramachandra Medical College & Research Institute, Sri Ramachandra University, Chennai, India
5 School of Biosciences & Technology, VIT University, Vellore, India
6 School of Medicine & Professor of Pediatrics, University of Colorado, Aurora Colorado, USA

Correspondence Address:
Dr Ramamurthy Mageshbabu
Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital & Research Centre, Sripuram, Vellore 632 055, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijmr.IJMR_241_16

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Background & objectives: Human parvovirus B19V (B19V) is known to be associated with erythema infectiosum commonly in children, aplastic crisis, especially in persons with underlying haemolytic disorders, hydrops fetalis in pregnancies and arthritis. This cross-sectional study was aimed to determine the presence of B19V infection in childhood febrile illnesses, association of B19V with arthropathies and in adult patients with end-stage renal disease (ESRD) on dialysis. The genetic diversity among the sequences was also analysed. Methods: A nested polymerase chain reaction (nPCR) assay was used for B19V DNA targeting VP1/VP2 region and used for testing 618 patients and 100 healthy controls. Phylogenetic analysis on nucleotide and amino acid sequences was carried out to compare our sequences with other Indian strains and global strains. Results: Among 618 samples tested, seven (1.13%) were found positive. The phylogenetic analysis revealed that all the seven sequences belonged to genotype 1 and showed low genetic diversity. The clustering pattern of seven sequences was similar both by nucleotide and by predicted amino acid sequences. The fixed effects likelihood analysis showed no positive or negatively selected sites. Interpretation & conclusions: Seven samples (4 from non-traumatic arthropathies, 2 from patients with ESRD and 1 from febrile illness patient) were found positive by nPCR. When our seven sequences were compared with global strains, the closest neighbour was other Indian strains followed by the Tunisian strains.

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