|Year : 2017 | Volume
| Issue : 7 | Page : 9-14
Characterization of antimicrobial resistance markers & their stability in Salmonella enterica serovar Typhi
Yashwant Kumar, Anshu Sharma, Kavaratty Raju Mani
National Salmonella & Escherichia Centre, Central Research Institute, Kasauli, India
|Date of Submission||21-Jun-2015|
|Date of Web Publication||4-Dec-2017|
National Salmonella & Escherichia Centre, Central Research Institute, Kasauli 173 204, Himachal Pradesh
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background & objectives: Typhoid fever is a major cause of morbidity and mortality in the developing countries including India. Resistance to multiple antimicrobial agents is an emerging global problem that has serious impact on the treatment of disease. There are many factors associated with the emergence of resistance. Most important of them is the acquisition and further transmission and spread of resistance markers among various bacterial species. Therefore, we conducted this study to characterize the resistance plasmids in terms of their transferability and stability among Salmonella enterica serovar Typhi.
Methods: Six multidrug-resistant S. Typhi isolates were evaluated for the stability and transfer of resistance markers. The resistance plasmids were also checked for the presence of RepHI1A replicon.
Results: All resistance markers were found to be transferred to the recipient through conjugation and transformation, except for nalidixic acid. None of the resistance plasmid was found to harbour RepHI1A replicon and therefore, did not belong to incompatibility group IncHI1. Resistance markers were found to be highly stable in all the isolates during serial passages and storage as stab cultures at different temperatures for different time periods.
Interpretation & conclusions: Resistance markers for chloramphenicol, ampicillin, streptomycin and trimethoprim were transferred through conjugation as well as transformation whereas that for nalidixic acid was not transferred in any of the isolates. Markers for chloramphenicol and streptomycin resistance were found to be most stable during various storage conditions. Presence of small-sized non-IncHI1 resistance plasmids is a matter of concern due to their capability to exist inside the host, thereby increasing the possibility of their transmission and spread among S. Typhi and other bacterial species.
Keywords: Resistance plasmids - Salmonella Typhi – stability - transmission
|How to cite this article:|
Kumar Y, Sharma A, Mani KR. Characterization of antimicrobial resistance markers & their stability in Salmonella enterica serovar Typhi. Indian J Med Res 2017;146, Suppl S1:9-14
|How to cite this URL:|
Kumar Y, Sharma A, Mani KR. Characterization of antimicrobial resistance markers & their stability in Salmonella enterica serovar Typhi. Indian J Med Res [serial online] 2017 [cited 2019 Dec 7];146, Suppl S1:9-14. Available from: http://www.ijmr.org.in/text.asp?2017/146/7/9/219469
Typhoid fever remains a major public health threat globally with over 21.6 million cases and 220,000 deaths occurring annually ,. Although typhoid fever is not common in the developed countries due to improved sanitation and hygiene, it remains an important and persistent health problem in the developing nations with highest incidence in India and Pakistan ,.
Emergence of antimicrobial resistance among Salmonella More Details enterica serovar Typhi (S. Typhi) is a major problem. Being the most effective drug, chloramphenicol was used for the treatment of typhoid fever until the emergence of chloramphenicol-resistant strains of S. Typhi in 1972,. Since 1989, emergence of multidrug-resistant (MDR) S. Typhi, resistant to chloramphenicol, ampicillin, trimethoprim, streptomycin, sulphamethoxazole and tetracycline, has been reported from the developing countries including India ,,. Therefore, fluoroquinolone class of antibiotics became the treatment of choice for enteric fever ,.
S. Typhi strains those were resistant to nalidixic acid and exhibited reduced susceptibility to quinolones have been reported subsequently in a number of countries . There have been several reports indicating the re-emergence of susceptibility to conventionally used drugs ,, which raise the possibility of their reintroduction into therapeutic regimen. Among S. Typhi, MDR is associated with self-transmissible IncHI1 plasmids, which carry a panel of genes conferring resistance to several first-line antimicrobials . From 1995 onwards, 98 per cent of MDR S. Typhi isolates were of haplotype H58, carrying the IncHI1 plasmid  and are widespread in Africa and Asia . In view of these observations, this study was undertaken to characterize the antimicrobial resistance in S. Typhi isolates in terms of the resistance markers which render the microorganism resistant to different classes of antibiotics.
| Material & Methods|| |
In our previous study , of the 128 isolates of S. Typhi received during the 2008-2009 at the National Salmonella and Escherichia More Details Centre, Central Research Institute, Kasauli, India, from all around the country, six were found to be MDR showing resistance to chloramphenicol, ampicillin, streptomycin, nalidixic acid and trimethoprim (C-A-S-Na-Tr), the MDR isolates were used in the present study for characterization of resistance markers. These were tested for the transfer of resistance through conjugation and transformation. The resistance plasmids were further tested to determine if they belonged to incompatibility group IncHI. Stability of resistance markers was also assessed during regular subcultures and storage at different temperatures for different time period.
Conjugation and transformation: Conjugation experiments were performed according to the method previously reported  to check the transfer of resistance from donor (each S. Typhi isolate) to recipient [CGSC 6576 (F −, λ−, recA1), IN(rrnD-rrnE)1, rpoB331 (rifampicin resistance [Rif R]), hsdR19], (provided by Dr John Wertz, Coli Genetic Stock Centre, Yale University, New Haven, CT, USA). For transformation, plasmid DNA was isolated from each S. Typhi isolate as previously reported . Transformation experiments were carried out using Escherichia coli HMS174 (F -recA1 hsdR(rK12− mK12+) (Rif R) competent cell kit (Novagen, Germany) as per the manufacturer's instructions.
PCR for RepHI1A replicon: Plasmid DNA isolated from each isolate was tested for the presence of RepHI1A replicon by PCR using the primers 5'-GGTCCAACCCATTGCTTTAC-3' and 5'-CACG GAAAGAAATCACAAC-3' as previously reported  on Mastercycler (Eppendorf AG, Germany). Briefly, PCR reaction was set up using 50 ng plasmid DNA and 50 nM of each primer in a buffer composed of 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 200 μM dNTP mixture and 1U of Taq polymerase in a final volume of 100 μl. Amplification conditions were thirty cycles of 94°C/30 sec, 55°C/30 sec and 72°C/30 sec, with a final extension step of 72°C for 10 min. The gel was observed for 365 bp amplicon suggestive of RepHI1A replicon as reported previously .
Stability of resistance plasmids during subcultures: Stability of the resistance markers in the test strains was determined as described . The resistance pattern and the plasmid content were determined before conducting the study. Briefly, each isolate was sub-cultured in 5 ml of pen assay broth (Becton-Dickinson, New Jersey, USA) and incubated at 37°C for overnight. Daily sub-culture was performed in fresh pen assay broth for 25 days, and stability of resistance pattern was determined intermittently on 5, 10, 15, 20 and 25 passage by antimicrobial susceptibility test by disc diffusion method .
Stability of resistance markers during storage: Stability of the resistance markers in each isolate was determined as described previously  after storage at 4°C, 22°C and 30°C for 1, 3, 6, 9 and 12 months. Each isolate was inoculated in Luria-Bertani (LB) broth and incubated at 37°C. From this, a series of identical stab cultures were prepared in Difco agar. The stab cultures were sealed with a cork soaked in heated, sterile Vaseline. These were stored at 4, 22 and 30°C. At varying intervals (after 1, 3, 6, 9 and 12 months), stab cultures were revived by inoculating in 0.5 ml of fresh LB broth and incubated overnight at 37°C and tested for any loss of resistance marker by disc diffusion susceptibility testing.
| Results|| |
All six isolates of S. Typhi transferred resistance markers for chloramphenicol, ampicillin, streptomycin and trimethoprim to the recipient by conjugation as well as transformation. However, resistance marker for nalidixic acid was not transferred from any of the tested isolates by either of the methods [Table 1].
All isolates were found to harbour a single plasmid of about 33.5 kb. During the detection of RepHI1A replicon, none of the isolates exhibited the presence of the same. Positive control (plasmid from strain CT18) produced a fine band of 365 bp showing the presence of RepHI1A replicon [Figure 1].
|Figure 1: Polymerase chain reaction for the detection of RepH1A replicon. All six isolates were found to be negative for the RepH1A replicon, whereas plasmid from the Salmonella enterica serovar Typhi CT18 produced a fine band of 365 bp representative of RepH1A replicon. L: Ladder, Lane 1-6: Test plasmid DNA, C: plasmid from Salmonella enterica serovar Typhi CT18.|
Click here to view
No loss of resistance markers was observed in 83.3 per cent of the isolates during serial passages up to 25th passage, except for loss of streptomycin resistance in one isolate at 25th passage [Table 2]. During storage of the isolates at different temperatures for different time periods, streptomycin was lost in one isolate on 12 months storage at 2-8°C whereas chloramphenicol and streptomycin were lost on 12 months storage at 30°C in another [Table 3].
| Discussion|| |
MDR in S. enterica serovar Typhi poses a major threat to the effective treatment of the disease and has been prevalent in India and the neighbouring countries since 1989,. In most cases, MDR is associated with high molecular weight and self-transferable plasmid transferring the phenotype individually or en bloc,.
In the present study, of five resistance markers, only four were found to be transferred both through conjugation and transformation. Nalidixic acid resistance marker was not transferred by either of the methods. Transfer of resistance phenotype through conjugation and transformation indicated plasmid-borne antibiotic resistance. The absence of transfer of nalidixic acid resistance may be due to its chromosomal origin . Quinolone resistance is mediated by non-transmissible, spontaneously occurring point mutations in chromosomal genes (gyrA, gyrB, parC and parE). These point mutations alter the enzymes, DNA gyrase and topoisomerase IV that are targets for quinolone drugs. The most frequent mutation is a single-point mutations in the gyrA gene, characteristically occurring at position 83 of the DNA gyrase enzyme (changing serine to phenylalanine) and position 87 (changing aspartate to tyrosine or glycine),.
The mechanism of fluoroquinolone resistance in S. enterica serovar Typhi is not completely understood and there have been only a few studies on fluoroquinolone resistance in this organism . A single mutation in the gyrA gene is sufficient to confer resistance to nalidixic acid and reduced susceptibility to fluoroquinolones, and a second mutation leads to high level fluoroquinolone resistance ,.
Plasmid-mediated quinolone resistance appears often to be co-transmitted with resistance to broad-spectrum β-lactmases, enabling the promulgation of simultaneous resistance to two classes of antimicrobial agents commonly used to treat serious Salmonella infections . The present study showed transferrable nature of the MDR, small-sized plasmid through conjugation and transformation, which might contribute to the spread of MDR among S. enterica serovar Typhi, leading to further development of complications in the treatment of typhoid fever.
Plasmids of different incompatibility groups have been found to persist among S. Typhi ,, but those of the incompatibility group IncHI1 appear to be particularly common in this serovar of S. enterica due to its transmission potential . Plasmids belonging to the incompatibility group IncH1 are frequently the source of resistance to ampicillin, chloramphenicol, trimethoprim and tetracycline. These plasmids are quite large ranging from 140-180 Kb . Conjugational transfer of these plasmids has been reported to be thermosensitive in nature being transferred at higher frequencies at ambient temperature (27°C) and not at an in vivo temperature (37°C).
In the present study, the absence of RepHI1A replicon was shown by non-amplification of the 365 bp region indicative of RepHI1A replicon. This exhibits that the R-plasmids do not belong to the incompatibility group IncH1. This finding is further supported by small size (33.5 kb) of the plasmid and no thermal sensitivity of the plasmid for conjugational transfer. Moreover, S. enterica serovar Typhi strain CT18, containing IncH1 plasmid, was used as a positive control and it gave a band of 365 bp amplicon. Some authors , have reported R-plasmids of S. enterica serovar Typhi belonging to the incompatibility group IncA, IncC and IncI. This finding reveals the emergence of non-IncHI1 conjugative plasmids having potential to transfer and spread of MDR among S. enterica serovar Typhi.
Plasmid profiling is a commonly used method of typing bacteria in epidemiological investigations. Therefore, it is pertinent to assess the influence of storage conditions on plasmid stability. Moreover, high stability of R-plasmids ensures the long-term persistence of the same in the bacterium and also increases the possibility of its spread through horizontal transfer.
Resistance plasmids were found to be stable during several serial passages, except loss of streptomycin resistance at 25th passage in one isolate. The same finding of partial loss of resistance due to loss of some of the resistance markers has also been reported previously . Streptomycin resistance was found to be more prone for loss whereas resistance genes for chloramphenicol, ampicillin and trimethoprim were found to be stable. The presence of highly stable R-plasmids in S. enterica serovar Typhi has also been reported previously from India . The stable nature of the R-plasmids may increase the chances of conjugational transfer thereby aiding in the spread and maintenance of MDR in S. enterica serovar Typhi. Moreover, due to stable nature of these R-plasmids, plasmid profiling can be used as a reproducible method for epidemiological investigations.
Presence of small-sized, highly stable, non-IncHI1 resistance plasmids among S. enterica serovar Typhi is of major concern due to its transmission potential further increasing the possibility of spread of antimicrobial resistance among this serovar. However, a well-planned and collaborated effort should be made by hospitals, surveillance centres and research institution to keep an eye on the current trends of antimicrobial resistance and the underlying mechanisms.
| Acknowledgment|| |
Authors thank the heads of all the laboratories that referred Salmonella isolates to this centre, and Shri Jiwa Ram for supplying media and biochemicals for biotyping. The technical assistance of Sarvshri Gian Chand and Khushnihal Kaushal is also acknowledged.
Conflicts of Interest: None.
| References|| |
Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bull World Health Organ
Kothari A, Pruthi A, Chugh TD. The burden of enteric fever. J Infect Dev Ctries
Kanungo S, Dutta S, Sur D. Epidemiology of typhoid and paratyphoid fever in India. J Infect Dev Ctries
Ochiai RL, Acosta CJ, Danovaro-Holliday MCD, Baiqing D, Bhattacharya SK, Agtini MD, et al.
A study of typhoid fever in five Asian countries: Disease burden and implications for controls. Bull World Health Organ
Centers for Disease Control. Typhoid fever-Mexico. Morb Mortal Wkly Rep
Anderson ES. The problem and implications of chloramphenicol resistance in the typhoid bacillus. J Hyg (Lond)
Threlfall EJ, Rowe B, Ward LR. Occurrence and treatment of multiresistant Salmonella
typhi. Public Health Lab Serv Microbiol Dig
Mandal BK. Treatment of multiresistant typhoid fever. Lancet
Prakash K, Pillai PK. Multidrug-resistant Salmonella
Typhi in India. APUA Newsletter
Bhan MK, Bahl R, Bhatnagar S. Typhoid and paratyphoid fever. Lancet
World Health Organization. Background document: The diagnosis, treatment and prevention of typhoid fever
. Geneva, Switzerland: Department of Vaccines and Biologicals, WHO; 2003.
Parry CM, Hien TT, Dougan G, White NJ, Farrar JJ. Typhoid fever. N Engl J Med
Krishnan P, Stalin M, Balasubramanian S. Changing trends in antimicrobial resistance of Salmonella enterica
serovar typhi and Salmonella enterica
serovar paratyphi A in Chennai. Indian J Pathol Microbiol
Nagshetty K, Channappa ST, Gaddad SM. Antimicrobial susceptibility of Salmonella
typhi in India. J Infect Dev Ctries
Wain J, Diem Nga LT, Kidgell C, James K, Fortune S, Song Diep T, et al.
Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella
serovar typhi strains associated with typhoid fever. Antimicrob Agents Chemother
Holt KE, Phan MD, Baker S, Duy PT, Nga TV, Nair S, et al.
Emergence of a globally dominant IncHI1 plasmid type associated with multiple drug resistant typhoid. PLoS Negl Trop Dis
Holt KE, Baker S, Dongol S, Basnyat B, Adhikari N, Thorson S, et al.
High-throughput bacterial SNP typing identifies distinct clusters of Salmonella
typhi causing typhoid in Nepalese children. BMC Infect Dis
Kumar Y, Sharma A, Mani KR. Antibiogram profile of Salmonella enterica
serovar typhi in India – A two year study. Trop Life Sci Res
Mills-Robertson F, Addy ME, Mensah P, Crupper SS. Molecular characterization of antibiotic resistance in clinical Salmonella
typhi isolated in Ghana. FEMS Microbiol Lett
Engebrecht J, Brent R, Kaderbhai MA. Minipreps of plasmid DNA. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, et al
., editors. Current protocols in molecular biology
. New York: John Wiley and Sons Inc.; 2003. p. 1.6.1-1.6.10.
Gabant P, Newnham P, Taylor D, Couturier M. Isolation and location on the R27 map of two replicons and an incompatibility determinant specific for IncHI1 plasmids. J Bacteriol
Shanahan PMA, Jesudason MV, Thomson CJ, Amyes SGB. Molecular analysis of and identification of antibiotic resistance genes in clinical isolates of Salmonella
typhi from India. J Clin Microbiol
Watanabe T, Ogata Y. Genetic stability of various resistance factors in Escherichia coli
and Salmonella typhimurium
. J Bacteriol
Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved standard-Seventh edition. M7-A7
. Wayne, PA: CLSI; 2006.
Olsen JE, Brown DJ, Baggesen DL, Bisgaard M. Stability of plasmids in five strains of Salmonella
maintained in stab culture at different temperatures. J Appl Bacteriol
Sabherwal U, Chaudhary U, Saini S. Multidrug-resistant Salmonella
typhi in Haryana in 1989-90. Indian J Med Res
Sudarsana J, Nair L, Devi KI. Multidrug resistant Salmonella
typhi in Calicut, South India. Indian J Med Res
Parkhill J, Dougan G, James KD, Thomson NR, Pickard D, Wain J, et al.
Complete genome sequence of a multiple drug resistant Salmonella enterica
serovar typhi CT18. Nature
Mirza S, Kariuki S, Mamun KZ, Beeching NJ, Hart CA. Analysis of plasmid and chromosomal DNA of multidrug-resistant Salmonella enterica
serovar typhi from Asia. J Clin Microbiol
Gaind R, Paglietti B, Murgia M, Dawar R, Uzzau S, Cappuccinelli P, et al.
Molecular characterization of ciprofloxacin-resistant Salmonella enterica
serovar typhi and paratyphi A causing enteric fever in India. J Antimicrob Chemother
Chiou CS, Lauderdale TL, Phung DC, Watanabe H, Kuo JC, Wang PJ, et al.
Antimicrobial resistance in Salmonella enterica
serovar typhi isolates from Bangladesh, Indonesia, Taiwan, and Vietnam. Antimicrob Agents Chemother
Ugboko H, De N. Mechanisms of antibiotic resistance in Salmonella
typhi. Int J Curr Microbiol Appl Sci
Renuka K, Kapil A, Kabra SK, Wig N, Das BK, Prasad VV, et al.
Reduced susceptibility to ciprofloxacin and gyrA
gene mutation in North Indian strains of Salmonella enterica
serotype typhi and serotype paratyphi A. Microb Drug Resist
Thamizhmani R, Bhattacharya D, Sayi DS, Bhattacharjee H, Muruganandam N, Ghosal SR, et al.
Emergence of fluoroquinolone resistance in Salmonella enterica
serovar typhi in Andaman and Nicobar Islands, India. Indian J Med Res
Dutta S, Das S, Mitra U, Jain P, Roy I, Ganguly SS, et al.
Antimicrobial resistance, virulence profiles and molecular subtypes of Salmonella enterica
serovars typhi and paratyphi A blood isolates from Kolkata, India during 2009-2013. PLoS One
Gay K, Robicsek A, Strahilevitz J, Park CH, Jacoby G, Barrett TJ, et al.
Plasmid-mediated quinolone resistance in non-typhi serotypes of Salmonella enterica
. Clin Infect Dis
Phan MD, Wain J. IncHI plasmids, a dynamic link between resistance and pathogenicity. J Infect Dev Ctries
Hampton MD, Ward LR, Rowe B, Threlfall EJ. Molecular fingerprinting of multidrug-resistant Salmonella enterica
serotype typhi. Emerg Infect Dis
Taylor DE. Thermosensitive nature of IncHI1 plasmid transfer. Antimicrob Agents Chemother
Rangnekar VM, Gadre SV, Mukerji S, Chitnis DS. Genetic stability of IncH1 and other incompatibility group R-plasmids in Salmonella
typhi. Indian J Med Res
[Table 1], [Table 2], [Table 3]