Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
  Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login  
  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 2053       

   Table of Contents      
CORRESPONDENCE
Year : 2016  |  Volume : 143  |  Issue : 1  |  Page : 112-113

Authors' response


1 Department of Endocrinology, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India
2 Department of Nephrology/Translational & Regenerative Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India
3 Department of Histopathology, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India
4 Department of Pharmacology, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India

Date of Web Publication14-Mar-2016

Correspondence Address:
Anil Bhansali
Department of Endocrinology, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 26997025

Rights and PermissionsRights and Permissions

How to cite this article:
Bhansali S, Kumar V, Saikia UN, Medhi B, Jha V, Bhansali A, Dutta P. Authors' response . Indian J Med Res 2016;143:112-3

How to cite this URL:
Bhansali S, Kumar V, Saikia UN, Medhi B, Jha V, Bhansali A, Dutta P. Authors' response . Indian J Med Res [serial online] 2016 [cited 2019 Dec 12];143:112-3. Available from: http://www.ijmr.org.in/text.asp?2016/143/1/112/178621

We thank Deepa Bhartiya [1] for her interest in our article and the opportunity to clarify a number of points from our work [2] .

The Figure of mesenchymal stem cells Figure 1A [2] , which we presented in our article was obtained at the time of adherence (P0) during the procedure of isolation/culturing of the mesenchymal stem cell. It is expected that some non-MSCs may also be present as these are derived from bone marrow. These cells were not characterized at P0 stage, as it has been previously reported that the expression of stemness marker and homogeneity of MSCs enhances with increasing passage number [3] . Therefore, the cultured cells were characterized after three passages for the expression of rat MSCs surface markers, and found high positivity of CD29 and CD54 (>99%) and absence of CD45 and CD34, indicating the negligible presence of non-MSCs cells. This shows that with prolonged culturing of MSCs, the numbers of non-specific cells are progressively reduced. Further, the isolated MSCs were shown to have high expression of CD29, which was absent in very small life embryonic (VSEL) stem cells [4] , substantiating that the cells used for transplantation were preferentially MSCs. We scrolled the images, which obtained at different days of MSCs culturing, but failed to find VSEL type cells as pointed out by the author [1] .

The mechanisms involved in improvement of glycaemic control after stem cells transplantation remains elusive. Existing data support the role of MSCs in regenerating the islet cells as well as facilitating the islet cell proliferation [5] . The role of VSELs in regeneration of ß-cells as a part of procedure of MSCs culturing was unlikely in our study as we administered preferentially cell population of MSCs, though the possibility of STZ-induced mobilization of endogenous VSELs into the islets cannot be excluded [6] . Further, it was difficult to conclude in our study whether trans-differentiation or fusion of labelled stem cells with ß-cells resulted in improved glycaemia due to experimental limitations.

Your suggestion of transplantation of stem cells directly into the pancreas rather than though rat-tail vein is impressive. This may be because direct transplantation of stem cells into the pancreas may be more effective to control hyperglycaemia than through peripheral route [7],[8] .

 
   References Top

1.
Bhartiya D. Is the improved function of streptozotocin treated pancreas truly due to transdifferentiation/fusion of transplanted MSCs? Indian J Med Res 2016; 143 : 111-2.  Back to cited text no. 1
    
2.
Bhansali S, Kumar V, Saikia UN, Medhi B, Jha V, Bhansali A, et al. Effect of mesenchymal stem cells transplantation on glycaemic profile & their localization in streptozotocin induced diabetic Wistar rats. Indian J Med Res 2015; 142 : 63-71.  Back to cited text no. 2
[PUBMED]    
3.
Ikebe C, Suzuki K. Mesenchymal stem cells for regenerative therapy: optimization of cell preparation protocols. Biomed Res Int 2014; 2014 : 951512.  Back to cited text no. 3
    
4.
Ratajczak MZ, Zuba-Surma EK, Ratajczak J, Wysoczynski M, Kucia M. Very Small Embryonic Like (VSEL) Stem Cells - Characterization, Developmental Origin and Biological Significance. Exp Hematol 2008; 36 : 742-51.   Back to cited text no. 4
    
5.
Hashemian SJ, Kouhnavard M, Nasli-Esfahani E. Mesenchymal stem cells: rising concerns over their application in treatment of type one diabetes mellitus. J Diabetes Res 2015; 2015 : 675103.  Back to cited text no. 5
    
6.
Bhartiya D, Patel H. Very small embryonic-like stem cells are involved in pancreatic regeneration and their dysfunction with age may lead to diabetes and cancer. Stem Cell Res Ther 2015; 6 : 96.  Back to cited text no. 6
    
7.
Bhansali A, Upreti V, Khandelwal N, Marwaha N, Gupta V, Sachdeva N, et al. Efficacy of autologous bone marrow derived stem cell transplantation in patients with type 2 diabetes mellitus. Stem Cells Dev 2009; 18 : 1407-16.  Back to cited text no. 7
    
8.
Bhansali A, Asokumar P, Walia R, Bhansali S, Gupta V, Jain A, et al. Efficacy and safety of autologous bone marrow derived stem cell transplantation in patients with type 2 diabetes mellitus: a randomized placebo-controlled study. Cell Transplant 2014; 23 : 1075-85.  Back to cited text no. 8
[PUBMED]    




 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    References

 Article Access Statistics
    Viewed410    
    Printed2    
    Emailed0    
    PDF Downloaded146    
    Comments [Add]    

Recommend this journal