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ORIGINAL ARTICLE
Year : 2015  |  Volume : 142  |  Issue : 5  |  Page : 538-542

Revisiting the susceptibility testing of Mycobacterium tuberculosis to ethionamide in solid culture medium


1 Department of Bacteriology, National Institute for Research in Tuberculosis (ICMR), Chennai, India
2 World Health Organization - India Office, New Delhi, India
3 Department of Statistics, National Institute for Research in Tuberculosis (ICMR), Chennai, India

Correspondence Address:
Vanaja Kumar
Centre for Drug Discovery & Development (3D), Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600 119, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0971-5916.171278

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Background & objectives: Increase in the isolation of drug resistant phenotypes of Mycobacterium tuberculosis necessitates accuracy in the testing methodology. Critical concentration defining resistance for ethionamide (ETO), needs re-evaluation in accordance with the current scenario. Thus, re-evaluation of conventional minimum inhibitory concentration (MIC) and proportion sensitivity testing (PST) methods for ETO was done to identify the ideal breakpoint concentration defining resistance. Methods: Isolates of M. tuberculosis (n=235) from new and treated patients were subjected to conventional MIC and PST methods for ETO following standard operating procedures. Results: With breakpoint concentration set at 114 and 156 µg/ml, an increase in specificity was observed whereas sensitivity was high with 80 µg/ml as breakpoint concentration. Errors due to false resistant and susceptible isolates were least at 80 µg/ml concentration. Interpretation & conclusions: Performance parameters at 80 µg/ml breakpoint concentration indicated significant association between PST and MIC methods.


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