Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
  Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login  
  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 1179       
ORIGINAL ARTICLE
Year : 2012  |  Volume : 136  |  Issue : 3  |  Page : 460-465

Effects of cow ghee (clarified butter oil) & soybean oil on carcinogen-metabolizing enzymes in rats


1 Division of Animal Biochemistry, National Dairy Research Institute, Karnal, India
2 Division of Animal Biochemistry, National Dairy Research Institute, Karnal and Present address: Director, Doon Valley Institute of Pharmacy & Medicine, Sector 17, Karnal 132 001, India

Correspondence Address:
Vinod K Kansal
Professor & Head, Division of Animal Biochemistry, National Dairy Research Institute, Haryana, Karnal 132 001
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 23041740

Rights and PermissionsRights and Permissions

Background & objectives: Our previous study showed that cow ghee relative to soybean oil had a protective effect against carcinogen induced mammary cancer in rats. The objective of this study was to elucidate its biochemical mechanism. Methods: Two groups of 21 day old rats (20 each) were fed for 44 wk diet containing cow ghee or soybean oil (10%). Five animals from each group were sacrificed at 0 day and at 5, 21 and 44 wk for analysis of phase I and phase II pathways enzymes of carcinogen metabolism. Results: Dietary cow ghee relative to soybean oil decreased the activities of cytochrome P450 (CYP) enzymes, CYP1A1, CYP1A2, CYP1B1 and CYP2B1, responsible for activation of carcinogen in liver. Carcinogen detoxification activities of uridinediphospho-glucuronosyl transferase (UDPGT) and quinone reductase (QR) in liver, and γ-glutamyltranspeptidase (GGTP) and QR in mammary tissue were significantly higher in cow ghee fed rats than in soybean oil fed rats. The hepatic GGTP activity decreased on soybean oil diet; while in cow ghee group it remained unaffected. Interpretation & conclusions: Our findings show that dietary cow ghee compared to soybean oil downregulates the enzyme activities responsible for carcinogen activation in liver and upregulates carcinogen detoxification activities in liver and mammary tissues.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1140    
    Printed32    
    Emailed0    
    PDF Downloaded487    
    Comments [Add]    

Recommend this journal