Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
  Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login  
  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 632       
ORIGINAL ARTICLE
Year : 2012  |  Volume : 136  |  Issue : 1  |  Page : 40-45

Inhibition of toxic actions of phospholipase A 2 isolated & characterized from the Indian Banded Krait (Bungarus fasciatus) venom by synthetic herbal compounds


1 Department of Physiology, University of Calcutta, Kolkata, India
2 Drug Development Diagnostics & Biotechnology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India
3 Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

Correspondence Address:
Antony Gomes
Scientist, Drug Development Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 032
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

Background & objectives: Phospholipase A 2 (PLA 2 ) is one of the major constituents of krait venom associated with several pathophysiological actions like myotoxicity, cardiotoxicity, neurotoxicity, etc. As there was no specific antiserum available against Bungarus fasciatus venom, this study was done with synthetic herbal compounds, anti PLA 2 rabbit antiserum and commercial polyvalent snake venom antiserum to neutralize the PLA 2 induced toxicities in experimental models. Methods: B. fasciatus venom phospholipase A 2 fraction 38 (BF-38) was isolated by ion exchange chromatography, molecular weight was determined by mass spectrometry and its N terminal amino acid sequence was identified. Monospecific rabbit antiserum was raised against the PLA 2 in presence of Freund complete adjuvant. The neutralization of PLA 2 induced toxicities was done in in vitro and in in vivo models using synthetic herbal compounds, anti PLA 2 rabbit antiserum and commercial polyvalent snake venom antiserum. Results: A toxic PLA 2 (BF-38) was purified from the B. fasciatus venom by CM-cellulose and HPLC, of 13.17 kDa and a minor band of 7.3 kDa using ESI-MS. The 13.17 kDa PLA 2 sequence was NLYQFKNMIQC. The 7.3 kDa toxin sequence was RKCLTKYSQDNES and was found to be <10 per cent w/w. Anti PLA 2 rabbit antiserum produced faint precipitant band in immunogel diffusion and showed low titre value. The commercial polyvalent snake venom antiserum, anti PLA 2 rabbit antiserum and the synthetic herbal compounds neutralized the PLA 2 induced toxicities at different intensities. Interpretation & conclusions: Our results suggested that synthetic herbal compound (BA) along with antiserum might provide effective protection against PLA 2 induced toxicities of B. fasciatus venom.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1132    
    Printed47    
    Emailed0    
    PDF Downloaded297    
    Comments [Add]    

Recommend this journal