Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
  Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login  
  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 6889       
ORIGINAL ARTICLE
Year : 2011  |  Volume : 134  |  Issue : 3  |  Page : 362-368

Mycobacterial interspersed repetitive unit typing in Mycobacterium tuberculosis isolates from Sichuan province in China


1 Department of Veterinary Medicine, Southwest University, Chongqing;College of Life Sciences, Sichuan University, Sichuan, China
2 College of Bioengineer, Xihua University, Sichuan, China
3 Department of Veterinary Medicine, Southwest University, Chongqing, China
4 Chengdu Antituberculosis Hospital, Sichuan, P.R, China
5 College of Life Sciences, Sichuan University, Sichuan, China

Correspondence Address:
Qun Sun
College of Life Sciences, Sichuan University, 29# Wangjiang Rd, Chengdu, Sichuan 610064, P.R
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 21985820

Rights and PermissionsRights and Permissions

Background & objectives : Emergence and spread of drug resistant Mycobacterium tuberculosis is a serious threat to tuberculosis (TB) control programme. Therefore, the objective of this study was to genotype drug-resistant M. tuberculosis strains isolated from patients in Sichuan, China, using Mycobacterial Interspersed Repetitive Units (MIRU) for epidemiological analysis. Methods: Drug-resistance testing of M. tuberculosis isolates from pulmonary TB patients was confirmed by proportion method. Twelve MIRU loci were analyzed on 80 drug-resistant and 9 susceptible isolates by polymerase chain reaction and agarose gel electrophoresis. Hunter-Gaston discriminatory index (HGI) values were determined for each 12 MIRU loci for the evaluation of their discrimination power. Results: Among 12 MIRU loci examined, polymorphic bands could be generated on 11 loci. Sixty five isolates had distinct MIRU patterns, while other 24 belonged to 8 clusters and resistant to at least one anti-TB drug tested. The association between the MIRU patterns and the mutation patterns of drug-resistance relevant target genes was not significant among the drug-resistant isolates. Interpretation & conclusions : The results showed that with a satisfactory discrimination power exhibited, the 12 loci based MIRU typing could be a valuable tool for epidemiological studies in M. tuberculosis isolates from Sichuan.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed847    
    Printed48    
    Emailed0    
    PDF Downloaded241    
    Comments [Add]    

Recommend this journal