Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
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Year : 2011  |  Volume : 134  |  Issue : 3  |  Page : 362-368

Mycobacterial interspersed repetitive unit typing in Mycobacterium tuberculosis isolates from Sichuan province in China

1 Department of Veterinary Medicine, Southwest University, Chongqing;College of Life Sciences, Sichuan University, Sichuan, China
2 College of Bioengineer, Xihua University, Sichuan, China
3 Department of Veterinary Medicine, Southwest University, Chongqing, China
4 Chengdu Antituberculosis Hospital, Sichuan, P.R, China
5 College of Life Sciences, Sichuan University, Sichuan, China

Correspondence Address:
Qun Sun
College of Life Sciences, Sichuan University, 29# Wangjiang Rd, Chengdu, Sichuan 610064, P.R
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Source of Support: None, Conflict of Interest: None

PMID: 21985820

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Background & objectives : Emergence and spread of drug resistant Mycobacterium tuberculosis is a serious threat to tuberculosis (TB) control programme. Therefore, the objective of this study was to genotype drug-resistant M. tuberculosis strains isolated from patients in Sichuan, China, using Mycobacterial Interspersed Repetitive Units (MIRU) for epidemiological analysis. Methods: Drug-resistance testing of M. tuberculosis isolates from pulmonary TB patients was confirmed by proportion method. Twelve MIRU loci were analyzed on 80 drug-resistant and 9 susceptible isolates by polymerase chain reaction and agarose gel electrophoresis. Hunter-Gaston discriminatory index (HGI) values were determined for each 12 MIRU loci for the evaluation of their discrimination power. Results: Among 12 MIRU loci examined, polymorphic bands could be generated on 11 loci. Sixty five isolates had distinct MIRU patterns, while other 24 belonged to 8 clusters and resistant to at least one anti-TB drug tested. The association between the MIRU patterns and the mutation patterns of drug-resistance relevant target genes was not significant among the drug-resistant isolates. Interpretation & conclusions : The results showed that with a satisfactory discrimination power exhibited, the 12 loci based MIRU typing could be a valuable tool for epidemiological studies in M. tuberculosis isolates from Sichuan.

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