Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
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REVIEW ARTICLE
Year : 2006  |  Volume : 124  |  Issue : 4  |  Page : 385-398

Applications of real-time PCR technology to mycobacterial research


National JALMA Institute for Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India

Correspondence Address:
V M Katoch
National JALMA Institute for Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India

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Source of Support: None, Conflict of Interest: None


PMID: 17159258

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Real-time polymerase chain reaction (PCR) is being used worldwide for various research purposes. In this review we discuss the principles of real-time PCR, different methodologies and chemistries available and their applications in mycobacterial research. This technology allows for the direct detection of PCR product during the exponential phase of the reaction. Being a very powerful, accurate, rapid and sensitive method, this technique holds immense promise for detection of mycobacteria, differentiation of mycobacterial species, quantification of mycobacterial load and detection of drug resistance in mycobacterial infection.


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